| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Ezakiella coagulans | TTTCGGACCGGTCACAGTG | ATGTCACAGCACCGACTCC | 59.93 | 59.71 | 275 | 162 |
100.00%
|
100.00%
|
| Primer 2 | Ezakiella coagulans | GGACAAGCTTGAAGCACACC | CGCCTCCAGGATTTGTGTG | 59.69 | 58.83 | 236 | 151 |
100.00%
|
100.00%
|
| Primer 3 | Ezakiella coagulans | GGACAAGCTTGAAGCACACC | TCGCCTCCAGGATTTGTGTG | 59.69 | 60.32 | 237 | 151 |
100.00%
|
100.00%
|
| Primer 4 | Ezakiella coagulans | TAACGAGGCCATGCTCACC | ACCGGACCTTGAAAGCAGG | 59.78 | 59.93 | 271 | 149 |
100.00%
|
100.00%
|
| Primer 5 | Ezakiella coagulans | GCAATTGGACAGGCGACTC | ACGAGCTTTGTGCCGTAGT | 59.20 | 59.63 | 191 | 143 |
100.00%
|
100.00%
|
| Primer 6 | Ezakiella coagulans | TTTGGACGGAACTGTCGGC | ATCGAGTGGAGCGTCATCC | 60.60 | 59.26 | 298 | 143 |
100.00%
|
100.00%
|
| Primer 7 | Ezakiella coagulans | TGCAATTGGACAGGCGAC | ACGAGCTTTGTGCCGTAGT | 58.33 | 59.63 | 192 | 141 |
100.00%
|
100.00%
|
| Primer 8 | Ezakiella coagulans | TTGCAATTGGACAGGCGAC | ACGAGCTTTGTGCCGTAGT | 59.04 | 59.63 | 193 | 141 |
100.00%
|
100.00%
|
| Primer 9 | Ezakiella coagulans | AATTGGACAGGCGACTCCC | ACGAGCTTTGTGCCGTAGT | 59.70 | 59.63 | 189 | 140 |
100.00%
|
100.00%
|
| Primer 10 | Ezakiella coagulans | ACACAGCAACCTTTGTGGGA | ACGAGCTTTGTGCCGTAGT | 60.03 | 59.63 | 154 | 140 |
100.00%
|
100.00%
|